Fig. 3.

^PaPBP1b-^PaLpoP complements the loss of aPBP activity in E. coli. Cells of E. coli MM10 (∆^EcponA P_ara::^EcponB) and its derivatives harboring the integrated plasmid expression constructs pMT116 (empty vector), pNG66 (P_lac::^PaponB-^PalpoP), pNG68 (P_lac:: ^PaponB), or pNG51 (P_lac::^PalpoP) were grown overnight in M9 medium with 0.2% arabinose at 37 °C. Cells were washed twice with M9 medium lacking a carbon source, normalized for cell density (OD₆₀₀ = 1), and serially 10-fold diluted (10⁻¹–10⁻⁶). Five microliters of each dilution was spotted onto M9 agar with or without the arabinose or IPTG inducers as indicated. Plates were incubated overnight at 37 °C and photographed.