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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Methods. 2017 Dec 13;137:37–48. doi: 10.1016/j.ymeth.2017.12.007

Figure 5. Surface immobilization strategies for smFRET experiments.

Figure 5

Mixture of biotin-PEG-NHS ester and PEG-NHS ester is covalently attached to amino-silanized slide surface. (A) S6Cy5/L9Cy3-labeled 70S ribosome is tethered to the quartz slide by hybridization of mRNA with a biotinylated DNA oligo bound to neutravidin [106]. (B) EF-G, domains II and IV of which are labeled with Cy3 and Cy5, is immobilized via C-terminal biotinylated BCCP tag [30].