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. Author manuscript; available in PMC: 2018 Sep 5.
Published in final edited form as: Nat Chem Biol. 2018 Mar 5;14(4):405–412. doi: 10.1038/s41589-018-0010-y

Figure 5. Global displacement of TRIM24 from chromatin and transcriptional response to TRIM24 degradation.

Figure 5

(a) Average genome-wide reads for TRIM24 centered on a ± 4 kb window around the TRIM24 peaks with the indicated treatment conditions (2.5 µM) for 24 hours. The y-axis shows ChIP-Rx signal (rpm/bp) and the x-axis indicates position. (b) ChIP-seq binding density of TRIM24 at exemplary asymmetrically loaded TRIM24 loci following the indicated treatments for 24 hours. The y-axis indicates ChIP-seq signal (rpm/bp) and the x-axis indicates genomic position. For a–b n=1 independently conducted experiment. (c) Gene expression change represented by Log2 fold change of transcripts versus Log10 p-value after 24 hours of 2.5 µM IACS-9571 treatment. Expression values were normalized to cell number using ERCC spike-in controls. (d) As described in (c), with dTRIM24 treatment. For c–d, data represents biological triplicates.