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. Author manuscript; available in PMC: 2019 Mar 30.
Published in final edited form as: J Mol Biol. 2018 Feb 6;430(7):1024–1050. doi: 10.1016/j.jmb.2018.01.021

Figure 4. UBE3A co-fractionates with HERC2 and NEURL4 in high molecular weight fractions in a size-exclusion experiment with SH-SY5Y cells protein extract.

Figure 4

8 mgs. of protein extract from SH-SY5Y cells were loaded on a Superose 6 column for size-exclusion chromatography. After fractionation, 20 µl of the collected fractions (500 µl) were analyzed by SDS-PAGE and Western blot using antibodies against the UBE3A, HERC2, NEURL4, PSMD4 and PSMA5. 20 µg of protein extract were loaded as input. The position where the peaks of protein standards eluted (669, 443, 200, 150, 66 and 29 kDa) and the void volume (V) are indicated over the fractions number.