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. Author manuscript; available in PMC: 2019 Mar 30.
Published in final edited form as: J Mol Biol. 2018 Feb 6;430(7):1024–1050. doi: 10.1016/j.jmb.2018.01.021

Figure 8. Confirmation of interaction of CAMK2D and CAMK2A with several HCIPs.

Figure 8

V5-tagged HCIPs were immunoprecipitated from T-REx 293 cells using anti-V5 magnetic beads. Protein extracts and immunoprecipitates were analyzed by SDS-PAGE and Western blot using antibodies against HA-tag, V5-tag and Actin. HA-SRC was used as negative control and V5-CAMK2B as positive control. The long black line indicates the place where part of the blots was removed because it was irrelevant for the figure. EV: empty vector.