Table 3.
Reciprocal immunoprecipitations of components of the high molecular weight complex as detected by mass spectrometry and CompPASS analysis.
| Prey | ||||||||
|---|---|---|---|---|---|---|---|---|
| UBE3A | HERC2 | NEURL4 | MAPK6 | ECH1 | ECI2 | HIF1AN | ||
| Bait | UBE3A | ++ | ++ | + | ++ | ++ | ++ | |
| HERC2 | ++a | ++b | − | − | − | − | ||
| NEURL4 | − | ++ | ++ | ++ | ++ | − | ||
| MAPK6 | − | ++ | ++ | + | + | − | ||
| ECH1 | − | − | − | − | − | − | ||
| ECI2 | − | + | + | − | ++ | − | ||
| HIF1AN | + | − | − | − | − | − | ||
+
Detected only in immunoprecipitations from T-Rex 293 cells.
++
Detected in immunoprecipitations from both T-Rex 293 and SH-SY5Y cells.
a
Using the fragment 4 of the HERC2 protein.