Figure 4.

ox‐LDL stimulates ENaC via LOX‐1‐mediated activation of NADPH oxidase. (A) Representative ENaC single‐channel currents recorded in two separate endothelial cells; one was pretreated with 125 μg·mL⁻¹ κ‐carrageenan before being treated with10 μg·mL⁻¹ ox‐LDL (upper trace); the other served as a control (Ctrl) and was treated with 125 μg·mL⁻¹ κ‐carrageenan (lower trace). (B) Representative ENaC single‐channel currents recorded in two separate endothelial cells; one was pretreated with 250 μM TEMPOL before the application of 10 μg·mL⁻¹ ox‐LDL (upper trace); the other served as a Ctrl and was pretreated with 250 μM TEMPOL (lower trace); (C) Representative ENaC single‐channel currents recorded in two separate endothelial cells; one was pretreated with 100 μM apocynin before the application of 10 μg·mL⁻¹ ox‐LDL (upper trace); the other served as a Ctrl and was treated with 100 μM apocynin (lower trace). Summarized ENaC P _Os were plotted on the right (n = 7 in each experiment).