RvE1 inhibits transendothelial migration of Mps in vitro. (A) The effect of RvE1 on migration of polarized Mps through a confluent HUVEC monolayer in a transwell assay. *P < 0.05, significantly different from corresponding PBS value; PBS, n = 7; RvE1, n = 7. (B) Relative mRNA levels for ChemR23 and BLT1 receptors in murine peritoneal Mps (n = 5). (C) Knockdown effects of siRNAs for BLT1 receptors (right side) and ChemR23 receptors (left side) in primary peritoneal Mps. Data are normalized to GAPDH levels. *P < 0.05, significantly different from corresponding scrambled value; Scramble, n = 7; Si‐ChemR23, n = 7; Si‐BLT1, n = 7). (D) Effect of silencing ChemR23 or BLT1 receptors on RvE1‐induced suppression of Mp trans‐endothelial migration in vitro. *P < 0.05, significantly different from vehicle; vehicle, n = 7; RvE1, n = 7. (E) Schematic diagram of RvE1‐mediated suppression on Ly6Chi and Ly6Clow Mos/Mps recruited in hearts after MI.