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. 2017 May 10;175(8):1305–1317. doi: 10.1111/bph.13817

Figure 6.

Figure 6

HS diet reduces eNOS phosphorylation and NO production. (A) Western blots of eNOS in isolated arteries with intact epithelium under indicated experimental conditions. (B) Summary of the levels of phosphorylated eNOS (p‐eNOS) after blocking ENaC with 0.5 μM amiloride (Ami) in SS rats fed with either NS or HS diet. (C) Western blots of Akt in isolated arteries with intact epithelium under indicated experimental conditions. (D) Summary of the levels of phosphorylated Akt (p‐Akt) after blocking ENaC with 0.5 μM amiloride in SS rats fed with either NS or HS. *P < 0.05, significantly different from NS; #P < 0.05, significantly different from HS; n = 6. (E) Intracellular NO levels detected with a membrane permeable fluorescent probe, DAF‐FMDA, under indicated conditions in endothelial cells. (F) Summary of fluorescence results from (E). *P < 0.05, significantly different from NS; #P < 0.05, significantly different from HS; n = 6.