Figure 1.

Gene editing of α‐gliadins in bread wheat. (a) Schematic of a typical α‐gliadin gene indicating the different protein domains. Two of the peptide sequences involved in gluten intolerance (p31‐43 and the 33‐mer) are represented by red arrows, whereas the target sequences for the sgRNAs (sgAlpha‐1 and sgAlpha‐2) are represented by blue arrows. Black arrows indicate primers used for Illumina sequencing. (b–d) Illumina sequencing of the α‐gliadin genes of 3 T1 BW208 mutant lines (T544, T545 and T553) transformed with sgAlpha‐2. (b) Alignment of the different deletion types found at the target locus of sgAlpha‐2; (c) Alignment of the different insertions at the target locus of sgAlpha‐2; and (d) frequency of the different type of insertions and deletions.