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. 2017 Nov 9;16(4):911–925. doi: 10.1111/pbi.12838

Figure 1.

Figure 1

Expression analysis of BnWRKY33 in leaves with the treatment of Sclerotinia sclerotiorum and salicylic acid (SA) as well as in different tissues. (a) Relative BnWRKY33 expression levels were quantified in Brassica napus (Ning RS‐1) using quantitative RT‐PCR at 0, 6, 12, 24 and 48 h after inoculation with S. sclerotiorum. Each fifth leaf of one‐month‐old plants was used for infection. (b) BnWRKY33 expression levels after treatment with SA. Each fifth leaf of three‐week‐old plants treated with SA was sampled at different time points and used for RNA purification. (c) Expression analysis of BnWRKY33 in different tissues including roots (RT) (roots from one‐week‐old Ning RS‐1 plants), stems (SM) (peduncle‐growing period), leaves (LF) (the third leaf of two‐week‐old plants), flowers (FR) (peduncle‐growing period), siliques (SE) (ten days after flowering), seedlings (SG) (one‐week‐old plants) and seeds (SD) (thirty days after flowering). The values and error bars indicate means ± standard error (n = 3).