Figure 3.

Effects of BnWRKY33 overexpression on the resistance and expression of defence‐related genes. (a) BnWRKY33 expression levels were quantified in transgenic plants and controls (Westar). (b) The lesions of detached leaves from T₂ plants were imaged at 48 h after inoculation. Scale bar, 2 cm. (c) Areas of lesions in the leaves were measured 48 h postinoculation. Three plants of transgenic and control lines each were used for assays. (d) Expression levels of camalexin synthesis‐related genes in BnWRKY33‐overexpressing lines (8‐5) and the control (Westar). (e) Expression difference of SA‐regulated genes between BnWRKY33‐overexpressing lines and the control. (f) Relative expression levels of JA‐regulated genes in BnWRKY33‐overexpressing lines and the control. The relative expression values were from three biological replicates. Single asterisks (*) indicate significance at P < 0.05 (Student's t‐test). Double asterisks (**) indicate highly significant differences (P < 0.01; Student's t‐test). The error bars indicate standard error.