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. 2017 Nov 9;16(4):911–925. doi: 10.1111/pbi.12838

Figure 4.

Figure 4

Identification of the Sclerotinia sclerotiorum‐responsive region in the promoter of BnWRKY33. (a) Sequence alignment of the promoter regions of BnWRKY33, AtWRKY33 and PcWRKY1. The numbers −346 and −246 indicate the truncated location in the promoter. The black box represents the W‐box region in the promoter. (b) Sclerotinia sclerotiorum‐induced β‐glucuronidase (GUS) expression in 1‐month‐old rosette leaves of transgenic Arabidopsis carrying the truncated promoters P‐346‐GUS and P‐249‐GUS at 24 h after inoculation with S. sclerotiorum. Scale bar, 2 mm. Similar results were obtained for three independent replicate experiments that involved the use of different transgenic lines.