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. 2017 Nov 9;16(4):911–925. doi: 10.1111/pbi.12838

Figure 5.

Figure 5

Yeast one‐hybrid assay for detecting specific interactive effects between 33box and BnWRKY15. The bait strain generated by integrating linearized 33box‐pAbAi into the genome of the Y1HGold yeast strain was transformed with the effector plasmid PGADT7‐Rec‐BnWRKY15 to validate the interaction. Y1HGold integrated with linearized empty pAbAi was transformed with the BnWRKY15 effector plasmid pGADT7‐Rec‐BnWRKY15 and served as a negative control. In addition, a positive control was generated by cotransforming the pGADT7‐Rec vector and p53 control into the Y1HGold, chromosome of which was integrated with the linearized p53‐pAbAi control plasmid. The normal growth of three Y1HGold yeast strains on SD/‐Leu plates in the absence of AbA indicated that the yeast growth status was healthy and unaffected by other factors (left), whereas only the bait strain containing the effector plasmid pGADT7‐Rec‐BnWRKY15 and the positive control showed growth on SD/‐Leu containing 1000 ng/mL aureobasidin A, suggesting specific interaction between BnWRKY15 and 33box (AbA; right).