Figure 9.

Transcriptional activation of BnWRKY15 and BnWRKY33 in Arabidopsis protoplasts. (a) Schematic representation of effector and reporter constructs used in Arabidopsis protoplast transient assays. The open reading frames of BnWRKY15 and BnWRKY33 were fused to the GAL4 DNA‐binding domain and used as effectors. The transcriptional activation abilities of AtERF4 and AtERF5, which were manifested as a transcriptional activator and a transcriptional repressor, respectively, served as controls. The GAL4 BD effector served as a negative control. (b) Relative luciferase activities were measured after cotransfection of protoplasts with different combinations of reporter and effector plasmids. The results represent the means ± standard errors (n = 3). Significantly different values (P < 0.05) according to Tukey's test (ANOVA) are marked with different letters.