Figure 11.

Influence of BnWRKY15 overexpression on the susceptibility of Brassica napus to Sclerotinia sclerotiorum. (a) Sclerotinia sclerotiorum resistance assays were performed using leaves detached from BnWRKY15‐overexpressing plants (T₀ line, 15OE‐4) and control (Westar) plants. (b) The expression levels of BnWRKY33, PAD3 and CYP71A13 between the T₀ line 15OE‐4 and the control (Westar) were compared using quantitative RT‐PCR. (c) To validate the heritability of resistance, two different T₁ lines (15OE‐2 and 15OE‐4) were used for resistance assays using detached leaves, and lesion areas were measured and imaged at 48 h after inoculation (n = 3). Asterisks (* and **) denote significant (P < 0.05) and highly significant (P < 0.01) differences between the transgenic line and the control (Westar), and the error bars indicate standard error (n = 3).