Figure 4.

PTEN was identified as a target gene of miR‐424‐3p. (A) PTEN mRNA 3′UTR was shown to have a complementary pairing sequence with miR‐424‐3p. (B) miR‐424‐3p mimics, inhibitor and scramble were transfected into A549 and H460 cells for 48 h, and Western blotting was performed to detect the effects of miR‐424‐3p on PTEN protein expression. (C) Dual‐luciferase reporter assay was used to determine the relative luciferase activity of A549 and H460 cells cotransfected with miR‐424‐3p mimics and pmirGLO‐Wt‐PTEN 3′UTR. *P < .05