Figure 6.

Overexpression of miR‐424‐3p or silencing of PTEN attenuates the effects of baicalein on A549 and H460 cells. Six groups were designed to determine the pathway of baicalein activity in A549 and H460 cells: Blank, baicalein treatment alone, miR‐424‐3p transfection alone, si‐PTEN transfection alone, baicalein with miR‐424‐3p transfection and baicalein with si‐PTEN transfection. (A) Expression levels of PTEN protein were detected in the six groups via Western blot. (B) Colony number was determined in the six groups via clone formation assay. (C) Apoptosis in cells was detected via Annexin V‐FITC/PI double staining and flow cytometry. (D) IC50 of cisplatin was calculated according to the OD value of cells when cotreated with different concentrations of cisplatin via CCK‐8 assay. *P < .05