Figure 4.

Effects of CB1R on gene expression depends on insulin receptor (IR). A, Western blot analysis of IR β‐subunit (IRβ) and GAPDH in βIRWT and βIRKO cells. B, Quantitative real‐time PCR analysis of CB1R expression in βIRWT and βIRKO cells. Data were normalized to 18S ribosomal RNA levels. C, Quantitative real‐time PCR analysis of CB1R, insulin, GCK and GLUT2 expression in βIRWT and βIRKO cells transfected with control (siCtrl) or CB1R (siCB1R) siRNA. D, Schematic unifying the regulation of β‐cell function by ECs and CB1Rs. Data are shown as the mean ± SEM from three independent experiments. *P < .05; **P < .01