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. 2018 Feb 7;22(4):2299–2310. doi: 10.1111/jcmm.13512

Figure 7.

Figure 7

miR‐141 promoted the expression of BDNF and dopaminergic neuron‐associated proteins (A) A protein network that interacts with the BDNF protein was identified using STRING: functional protein association networks. (B) The STRING tool revealed that BDNF directly promoted the expression of SHH and indirectly promoted the expression of TH and FBF8. (C) and (D) Comparison with the results of the gene chip assay revealed that except for NTF3, NGFR and CDC42, the mRNA levels of proteins that interact directly or indirectly with the BDNF protein were all significantly increased in iDNLCs cells. (E) Western blot analysis showed that the expression levels of FGF, SHH, NTRK3, CREB1, SORT1, NTRK1 and NTF4 in iDNLCs derived from SPIONs@miR‐141‐HuAESCs were significantly higher than the corresponding levels in HuAESCs; **P < 0.01 vs. HuAESCs, n = 3; *P < 0.05 vs. HuAESCs, n = 3; # P > 0.05 vs. HuAESCs, n = 3. (F) In iDNLCs derived from SPIONs@miR‐Mut‐HuAESCs, the expression levels of four factors (FGF, SHH, NTRK3 and CREB1) were significantly higher than those in HuAESCs.