A. Growth rates of the wild type, ΔLBBD mutant and LBBDcomp strains. The growth rate slopes of each strain are not statistically different from wild type as determined by linear regression analysis.
B. Growth rates of the the lbbD expressing strain SV1LBBD, the control strain SV1 containing pLe1SVK only, and cells that had lost the expression plasmid (SV1LBBD*) compared to wild type. Data represent the mean ± SD calculated from triplicate independent cultures. Wild type and SV1LBBD* strains were grown in the absence of antibiotic selection, whereas the other strains were grown in the presence of kanamycin. The growth rate slopes of wild type and SV1LBBD* are not statistically different from one another, but those of SV1 and SV1LBBD are different from wild type (p=0.0016), as determined by linear regression analysis.