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(A,B) CMO inhibits the phosphorylation of IκB and depletes the nuclear pool of p65 in hepatocellular carcinoma cells. HepG2 cells were treated with 25 µM CMO for indicated time point, after which, the cytoplasmic and nuclear extracts were prepared, and protein was resolved on SDS-PAGE gel, electrotransferred onto nitrocellulose membranes, and probed for phospho-IκB, IκB, phospho-p65, p65, GAPDH, and Lamin B antibodies.
