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. 2018 Mar 19;8:42. doi: 10.3389/fonc.2018.00042

Figure 5.

Figure 5

(A) Knockdown of p65 by small interfering RNA (siRNA) reduces the apoptotic effect of CMO. HepG2 cells were transfected with either control or p65 specific siRNA (50 nM). After 48 h, the cells were treated with CMO (25 or 50 µM) for 24 h, and the enzymatic activity of caspase-3/7 was determined by Caspase-Glo® 3/7 assay kit. (B) CMO increases the cleavage of PARP and Caspase 3 in HCCLM3 cells. HCCLM3 cells were treated with 50 µM CMO for 12, 24, 36, and 48 h, after which, the whole-cell extracts were prepared, and 30 µg of protein was resolved on 12% SDS-PAGE gel, electrotransferred onto nitrocellulose membranes, and probed for cleaved PARP and cleaved caspase 3 antibodies. The data are expressed as mean ± SD, compared with the untreated control (*p < 0.05).