Figure 2.

Acetylcholinesterase inhibition reduces bacterial load and prevents splenomegaly in orally infected mice without altering intestinal IgA production. Following pretreatment with saline or paraoxon, mice were infected orally with SL1344, as in Figure 1. (A). Bacterial colony-forming units (CFUs) were determined in spleen, liver, mesenteric lymph nodes (MLNs), and feces at the indicated time points postinfection and expressed as CFUs/mg. (B) Spleen weights of infected mice. (C) Total fecal IgA levels and (D) specific fecal anti-Salmonella IgA levels were determined by ELISA. Graphs depict the mean values ± SEM of data from three independent experiments (n = 4–10/group). Asterisks denote statistically significant differences between control and experimental groups (*p < 0.05, **p < 0.01).