Figure 1.

Catalpol inhibited Amyloid-β (Aβ) generation and secretion in Swedish mutant APP overexpressed N2a (SweAPP N2a) cells. (A) SweAPP N2a cells were treated with catalpol for 18 h, at the concentrations of 200 and 400 μM, respectively. Cell viability was detected by MTT assay. The results showed that catalpol has no significant effect on cell viability. (B) Immunoblot images and (C) quantifications showed that catalpol did not affect the expression levels of APP695. (D) ELISA analysis showing that catalpol was able to decrease Aβ1–42 levels in the conditional culture medium after treatment for 18 h. The data are expressed as the mean ± standard error of the mean (SEM). n = 3. The p values were calculated using one-way ANOVA.