Figure 2.

Catalpol did not affect the protein levels of β- and γ-secretase in SweAPP N2a cells. (A–F) SweAPP N2a cells were treated with catalpol (200 and 400 μM) for 18 h. Western blot was carried out to detect the expression levels of β-secretase (BACE-1) and the subunits of γ-Secretase (PS1, APH-1, PEN-2 and Nicastrin). (A) Immunoblot analysis and (B–F) quantifications showed catalpol did not show significant effect on the expression of the detected proteins related to amyloidogenic amyloid precursor protein (APP) proteolytic processing. The data are expressed as the mean ± SEM. n = 3. The p values were calculated using one-way ANOVA.