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. 2018 Mar 19;10:66. doi: 10.3389/fnagi.2018.00066

Figure 6.

Figure 6

Catalpol promoted ADAM10 expression through ERK/CREB signaling pathway. SweAPP N2a cells were pre-treated with the ERK inhibitor U0126 for 2 h, and then treated with 200 μM catalpol for 1 h. (A,C) Immunoblot images and (B,D) quantifications show that the expression levels of p-ERK/ERK and p-CREB/CREB were assessed by western blot. The expression levels of p-ERK and p-CREB were markedly decreased in cells treated with both U0126 and catalpol, compared with catalpol-treated cells. (E,F) SweAPP N2a cells were pre-treated with U0126 for 2 h, followed by treating with 200 μM catalpol for 18 h. Cells treated with both U0126 and catalpol showed significant decreased levels of ADAM10 protein (E, representative pictures; F, quantification), compare to these from catalpol-treated cells. The data are expressed as the mean ± SEM. n = 3. The p values were calculated using one-way ANOVA.