Figure 2.

Mutational trajectories. Order of appearance of mutations in the selected genetic elements: fhuA, fusA, and atp, cyo, and potABCD operons. Mutations in fusA where identified from a gentamicin concentration of 0.5 μg/ml in MG1655 and its ΔdnaQ derivative, while mutations in the cyo and potABCD operons where detected from an antibiotic concentration of 4 and 0.5 μg/ml in MG1655 and ΔdnaQ, respectively. Genetic modifications in fhuA were identified from a gentamicin concentration of 8 and 2 μg/ml in MG1655 and ΔdnaQ, respectively, while mutations on the atp operon were detected from a gentamicin concentration of 8 μg/ml in both ancestors.