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. 2018 Mar 19;9:494. doi: 10.3389/fmicb.2018.00494

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Copyright © 2018 Campodónico, Rifat, Chuang, Ioerger and Karakousis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mycobacterium tuberculosis RpoB mutation H526D is associated with altered bacillary length and cell wall thickness. The bacillary length (A) and cell wall thickness (B) of the RpoB H526D mutant and its parental strain were evaluated by transmission electron microscopy (TEM) during mid-log-growth-phase (7H9) and after exposure to nutrient starvation for 14 days (14NS). Bars, (A) 2 μm and (B) 100 nm. Dot plot graphs of (C) bacillary length (μm) and (D) cell wall thickness (nm). Each dot represents a measurement for a single bacillus. ^∗∗∗∗p < 0.0001, ^∗∗p < 0.01, ns: non-significant.