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RpoB mutation H526D alters bacterial cellular metabolic activity. The RpoB H526D mutant and parental strains were grown to mid-log-phase in nutrient-rich broth (7H9) or were starved of nutrients for 14 days (14NS) prior to incubation with AlamarBlue for 18 h. The fluorescence signal was normalized based on bacterial density. RFI, relative fluorescence intensity. ∗p < 0.05, ∗∗p < 0.01.
