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. 2018 Mar 19;9:501. doi: 10.3389/fmicb.2018.00501

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Copyright © 2018 Guo, Wang, Wang, Wang, Chen, Wu, Chen, Liu and Feng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Results of multiplex Luminex-based array. (A) Multiplex PCR of type strains representing each PSgc type. M, DNA Marker I (600, 500, 400, 300, 200, and 100 bp); 1, G2351; 2, G5305; 3, G5306; 4, G5307; 5, G5308; 6, G5310; 7, G5313; 8, G5319. (B) Specific detection based on the Luminex-based array. Biotin-labeled PCR products were separated by probe-coupled beads, and the hybridization signals are presented in terms of median fluorescence intensity (MFI) on the y-axis. Each sample representing the corresponding type strain is indicated on the x-axis. Data are combined from three independent experiments with error bars representing the standard deviation.