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. 2017 Nov 8;52(6):359–367. doi: 10.1136/bjsports-2017-098161

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This is an Open Access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 4.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited. See: http://creativecommons.org/licenses/by/4.0/

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Expression of markers of inflammation in cultured tendon-derived stromal cells after cytokine treatment. Tendon stromal cells were derived from healthy hamstring (n=6 donors), tendinopathic (n=6 donors) or ruptured Achilles (n=6 donors). (A) PDPN expression after IL-1β treatment (10 ng/mL) for 24 hours determined by flow cytometry. (B) Expression of PDPN mRNA after IL-1β treatment (10 ng/mL) for 24 hours. Expression of interferon target genes IRF5 (C) and IRF1 (D) after IFNγ treatment (20 ng/mL) for 24 hours. Gene expression is shown normalised to β-actin; bars represent median values. **P<0.01, *P<0.05. mRNA, messenger RNA; PDPN, podoplanin.