Figure 2.

Comparison of the global CD4+ and CD8+ TRBV repertoires in brain lesions and their presence in peripheral CD4+ and CD8+-ory T cell pools. (A) Logarithmic scatter plots comparing the number of copies of each CD4+ (left scatters), CD8+ (middle scatters), or undetermined (right scatters) clonotypes identified by gDNA sequencing and shared by each pair of brain lesions. CD4+ clonotypes are represented by triangles, CD8+ by squares and undetermined clonotypes by circles. Clonotypes not shared by lesions are shown in gray and shared ones in black. Large symbols represent clonotypes shared by the three lesions. Actual and normalized numbers of shared clonotypes as well as correlation coefficient (r) and coefficient of determination (r²) of their frequencies are shown in all scatters. (B) Charts representing the frequencies of CD4+ (in gray), CD8+ (in black), and undetermined, i.e., not identified in the peripheral pools, (in white) clonotypes in the global T cell repertoires and shared by each pair of brain lesions. (C) Dot plots comparing the numbers of copies in CD4+ (triangles, left graph) and CD8+ (squares, right graph) peripheral memory T cell pools of: all mem-CD4+ and mem-CD8+ peripheral clonotypes (all, white) and peripheral clonotypes also present in one, two or the three brain lesions (black). Large symbols represent clonotypes shared by the three lesions. Mean (gray line) and statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001) are shown. Frequencies of mem-CD4+ and mem-CD8+ clonotypes present in one, two, or three lesions are also shown.