Activation of the Wnt signaling pathway attenuates the inhibitory effects of rapamycin on PQ-induced EMT. (A) Western blot analysis of the EMT markers E-cadherin and α-SMA. (B) The western blot results of E-cadherin and α-SMA were quantified. (C) Reverse transcription-quantitative polymerase chain reaction was performed to measure the mRNA expression levels of E-cadherin and α-SMA. *P<0.05, **P<0.01 and ***P<0.001 as indicated. PQ, paraquat; EMT, epithelial-mesenchymal transition; SMA, smooth muscle actin; LiCl, lithium chloride.