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. 2018 Mar 1;38(3):101–110. doi: 10.1089/jir.2017.0038

FIG. 1.

FIG. 1.

Poly (I:C) induces IL-27 mRNA expression and secretion in uterine epithelial cells (A, B) and stromal fibroblasts (C, D). Confluent monolayers of epithelial cells and fibroblasts were treated with 25 μg/mL of poly (I:C). mRNA (A, C) and secretions (B, D) were recovered after 24 and 48 h, respectively, and levels of IL-27 measured by RT-PCR and ELISA (n = 4–5). For type I interferon (IFN) receptor complex (IFNAR) blockade (E, F), both epithelial cells and fibroblasts were pretreated with αIFNAR2 or IgG control (ISO) for 1 h before poly (I:C) treatment for the following 24 h. αIFNAR2 was maintained in cell culture media throughout the experiment (n = 3). mRNA expression is normalized to levels of β-actin. *P < 0.05; **P < 0.01; ***P < 0.001.