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. 2018 Mar 1;38(3):101–110. doi: 10.1089/jir.2017.0038

FIG. 5.

FIG. 5.

Estradiol inhibits IL-27-induced IDO expression through ERα in uterine stromal fibroblasts. Confluent monolayers of matched epithelial cells (A, B) and fibroblasts (C, D) were pretreated with estradiol (E2) at 5 × 10−8 M for 48 h before washout and subsequent treatment with E2 or IL-27 (100 ng/mL) for 24 h, after which mRNA expression levels for APOBEC3G (A3G) and IDO were measured by RT-PCR (n = 4–5). For ER blockade, Rx was added to confluent monolayers of fibroblasts (E) at 5 × 10−6 M for 1 h before addition of E2 at 5 × 10−8 M for a further 48 h, followed by washout and treatment with E2 or IL-27 (100 ng/mL) for a further 24 h. Rx was maintained in the media at 5 × 10−6 M for the entire duration of the experiment (n = 3). *P < 0.05. ER, estrogen receptor; Rx, Raloxifene.