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. Author manuscript; available in PMC: 2018 Mar 26.
Published in final edited form as: Methods Cell Biol. 2017 Jul 14;141:45–64. doi: 10.1016/bs.mcb.2017.06.005

FIG. 2.

FIG. 2

Sucrose step gradient enrichment of tau oligomers from an arachidonic acid-induced tau aggregation reaction sample. (A) The typical arachidonic acid-induced preparation produces a mixed population of long, intermediate, and short filaments as well as globular oligomeric structures. (B–F) The mixed population of aggregates can be separated over a sucrose step gradient consisting of the (B) sample (0% sucrose, F1), (C) 20% sucrose (F2), (D) 30% sucrose (F3), (E) 40% sucrose (F4), and (F) 50% sucrose (F5). Note that fraction 3 containing 30% sucrose contains a highly enriched population of oligomeric tau structures with very little to no contamination of filamentous structures. The short filaments and longer filaments are isolated in the 40% and 50% sucrose fractions, respectively. Scale bars are 200nm.