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. Author manuscript; available in PMC: 2018 Mar 26.
Published in final edited form as: Methods Cell Biol. 2017 Jul 14;141:45–64. doi: 10.1016/bs.mcb.2017.06.005

FIG. 3.

FIG. 3

Total and oligomeric tau sandwich ELISAs of 30% sucrose fraction from the sucrose step gradient enrichment of tau oligomers. (A) A representative Tau5 sELISA (total tau) with the monomeric and tau aggregates (arachidonic acid-induced) samples and the corresponding 30% sucrose fractions (F3) from the monomer and aggregate samples run over the sucrose step gradient. (B) A representative TOC1 sELISA (oligomeric tau) with the monomeric and tau aggregates (arachidonic acid-induced) samples and the corresponding 30% sucrose fractions (F3) from the monomer and aggregate samples run over the sucrose step gradient. Note the strong TOC1 reactivity in the 30% sucrose fraction from the tau aggregate fractionation procedure, which confirms the presence of oligomeric tau in this fraction. The absorbance values were converted to percent light absorbed as described in the text.