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Total GSH levels in granulomas from healthy individuals infected with BCG. GSH assay was performed using a colorimetric assay kit from Arbor Assays. Corrections were made to total protein measured by BCA Protein Assay Kit from Thermo Scientific. Both the GSH and total protein levels were measured in lysates excluding any supernatants from samples. There was a notable increase in the levels of GSH in L-GSH-treated granulomas. Data represent means ± SE from eight healthy individuals.
