Figure 16.
Survival of M. tb in untreated granulomas of healthy individuals. All samples were separated through density dependent centrifugation from peripheral blood of volunteers and PBMCs were isolated after washes with PBS. In each category 6 × 104 bacteria and 6 × 105 immune cells were used for an MOI of 0.1:1. CFU counts of granulomas formed from healthy individuals’ PBMCs showed a statistically significant increase at 15 days when left untreated. Data represent means ± SE from eight healthy individuals. *** p < 0.0005 when comparing samples at eight days and 15 days.