Figure 17.

(A) Quantification of M. tb within acidified compartments in untreated granulomas of healthy individuals. GFP-labeled M. tb was used for infection. Granulomas were labeled with lysotracker red DND99, which gets trapped within the acidified compartments, as well as DAPI for nucleus staining. When untreated, at 15 days granulomas showed significantly less M. tb in acidified compartments compared to the bacteria present in non-acidified compartments; (B) Untreated granulomas of T2DM individuals. Yellow areas correspond to acidified compartments with GFP-labeled bacteria present. Data represent means ± SE from eight healthy individuals. * p < 0.05 when comparing bacterial numbers in acidified versus non-acidified compartments. ** p < 0.005 when comparing bacterial numbers in acidified versus non-acidified compartments.