Figure 18.
(A) Survival of M. tb in untreated and L-GSH granulomas of healthy individuals. All samples were separated through density dependent centrifugation from peripheral blood of volunteers and PBMCs were isolated after washes with PBS. In each category 6 × 104 bacteria and 6 × 105 immune cells were used for an MOI of 0.1:1. CFU counts of granulomas formed from healthy individuals showed a statistically significant decrease at 15 days when treated with L-GSH; (B) Survival of M. tb in granulomas of T2DM individuals. All samples were separated through density-dependent centrifugation from peripheral blood of volunteers and PBMCs were isolated after washes with PBS. In each category 6 × 104 bacteria and 6 × 105 immune cells were used for an MOI of 0.1:1. CFU counts of granulomas formed from individuals with T2DM showed a statistically significant decrease at 15 days when treated with L-GSH. Data represent means ± SE from eight healthy individuals and six T2DM individuals. *** p < 0.0005 when comparing samples left untreated and treated with L-GSH for 15 days. * p < 0.05 when comparing untreated samples with samples treated with L-GSH at 15 days.