Figure 19.

(A) Quantification of Erdman within acidified compartments in PBMCs of healthy individuals treated with L-GSH. Green fluorescent protein (GFP)-labeled M. tb was used with lysotracker red DND99, which labeled acidified compartments, as well as DAPI for nucleus staining. When treated with L-GSH, granulomas showed an increase in bacterial number in acidified compartments compared to the bacteria present in non-acidified compartments. Yellow areas correspond to acidified compartments with GFP-labeled bacteria; (B) fluorescent staining of granulomas from T2DM individuals infected with M. tb. GFP-labeled M. tb was used with lysotracker red DND99, which labeled acidified compartments, as well as DAPI for nucleus staining. When treated with L-GSH, granulomas showed increased numbers of M. tb in acidified compartments compared to the bacteria present in non-acidified compartments. Yellow areas correspond to acidified compartments with GFP labeled bacteria present. Data represent means ± SE from five T2DM individuals and eight healthy individuals. * p < 0.05 when comparing bacterial numbers in acidified versus non-acidified compartments.