Figure 20.

Total GSH levels in granulomas from healthy individuals infected with M. tb. GSH assay was performed using a colorimetric assay kit from Arbor Assays. Corrections were made to total protein measured by BCA Protein Assay Kit from Thermo Scientific. Both the GSH and total protein levels were measured in lysates excluding any supernatants from samples. Once treated with L-GSH, granulomas from healthy individuals had a statistically significant increase in cellular levels of GSH. Data represent means ± SE from eight healthy individuals. ** p < 0.005 when comparing GSH levels in control and L-GSH treated groups.