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. 2018 Mar 5;7(3):46. doi: 10.3390/jcm7030046

Figure 4.

Figure 4

(a–c) Double immunostaining of the dorsal funiculus from the amiloride group at day 56 for BrdU (red) and adenomatous polyposis coli (APC) (green) with DAPI nuclear counterstaining (blue). The arrowheads indicate the BrdU- and APC-positive cells (scale bars 50 μm); (d) In both the amiloride and PBS groups, the number of BrdU- and APC-positive cells increased after SCI. Significantly more BrdU- and APC-positive cells were observed in the amiloride group than in the PBS group on day 56. The asterisk indicates significant difference between the amiloride and PBS groups with a Mann–Whitney U test (*p < 0.05, n = 5 per group, error bars are standard deviations).