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(a–e) Western blotting was performed using anti- myelin basic protein (MBP) (18, 23 kDa) and anti-b actin (45 kDa) antibodies. At days 28 and 56 after injury, MBP expression was significantly higher in the amiloride group compared to the PBS group. Asterisks indicate significant difference between the amiloride and PBS groups with a Mann–Whitney U test (* p < 0.05, n = 5 per group, error bars are standard deviations).
