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. 2018 Mar 12;2018:7863412. doi: 10.1155/2018/7863412

Figure 2.

Figure 2

Transmission electron microscopy of urinary extracellular vesicles. Four mls of urine was used to isolate EVs by Amicon ultrafiltration (MW cutoff = 100,000 kD). EVs were fixed in 2.5% glutaraldehyde in 0.1 M cacodylate buffer. Samples were stained with 1% osmium tetroxide in 0.1 M cacodylate buffer and subsequently stained with 0.5% aqueous uranyl acetate. A JEOL 1200EX transmission electron microscope (JEOL, Peabody, MA) was used for observation and photography. 1A. EVs from HIV-1 posi.