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. 2018 Mar 1;16(3):77. doi: 10.3390/md16030077

Figure 2.

Figure 2

ATG5+/+ cells are more vulnerable to coibamide A than ATG5−/− cells. (A) Morphological evaluation of wild-type and ATG5-null mouse embryonic fibroblasts (MEFs) in the presence of coibamide A (3 nM) or vehicle (0.1% DMSO). MEFs were treated as indicated for 24 h (panels a–d) or 36 h (panels e–h) and observed using light microscopy. Scale bar = 500 µm. (B) Trypan blue exclusion test of cell viability in wild-type and ATG5-null MEFs. Cells were seeded at 3000 cells/well and collected up to 48 h after treatment. Trypan Blue exclusion profiles represent mean cell counts ± SE of a time course performed in triplicate with the viability of vehicle-treated cells defined as 100%.