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. 2018 Mar 1;16(3):77. doi: 10.3390/md16030077

Figure 9.

Figure 9

Time-dependent analysis of autophagy and apoptosis signals in ATG5+/+ cells exposed to coibamide A or apratoxin A. Wild-type mouse embryonic fibroblasts (MEFs) were treated with, or without (0 unit), coibamide A (30 nM), apratoxin A (30 nM), or vehicle (Veh; 0.1% DMSO) for up to 30 h. Whole cell lysates were collected from adherent and detached (Det) cells (24 and 30 h). (A) Immunoblot analysis of ATG5 (detected in the context of the covalent ATG5-ATG12 complex), P62/SQSTM1, LC3, PARP1, caspase 3 expression relative to beta-actin. (B) Quantitation of immunoblot data shown in (A). Bars represent intensity of bands normalized to beta-actin, relative to vehicle-treated cells (open bars) in three independent experiments. Statistical significance is indicated as * p < 0.05 and ** p < 0.01.