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. 2018 Mar 8;16(3):83. doi: 10.3390/md16030083

Table 1.

Comparison of chitinolytic enzyme, protease and αGI production by various bacteria using deCSP as C/N source.

Strain Activity (U/mL)
Exochitinase Chitinase Chitosanase Protease aGI
Paenibacillus macerans TKU029 - 0.118 + 0.027 0.122 + 0.035 0.925 + 0.032 62.90 + 6.755
P. mucilaginosus TKU032 - 0.072 + 0.016 0.086 + 0.021 1.366 + 0.045 178.38 + 10.312
Paenibacillus sp. TKU037 - 0.135 + 0.018 0.069 + 0.019 1.203 + 0.041 180.77 + 11.620
Paenibacillus sp. TKU042 - 0.182 + 0.036 0.088 + 0.015 1.257 + 0.038 185.45 + 3.085
Bacillus licheniformis TKU004 - - 0.089 + 0.022 0.121 + 0.023 91.29 + 8.692
Bacillus subtilis TKU007 - 0.163 + 0.023 - 0.178 + 0.011 96.06 + 7.008
Lactobacillus paracasei TKU010 - 0.216 + 0.033 - 0.113 + 0.009 -
Serratia marcescens TKU011 - 0.201 + 0.029 0.149 + 0.021 0.115 + 0.012 73.63 + 4.090
Serratia ureilytica TKU013 0.276 + 0.012 0.266 + 0.018 0.099 + 0.017 0.202 + 0.024 64.52 + 3.474
Pseudomonas tamsuii TKU015 - 0.193 + 0.030 0.105 + 0.013 0.103 + 0.008 51.54 + 4.627
Serratia sp. TKU016 0.152 + 0.021 0.104 + 0.012 0.110 + 0.022 0.101 + 0.007 68.962.292
Serratia sp. TKU020 0.121 + 0.016 0.102 + 0.015 0.151 + 0.013 0.099 + 0.009 73.63 + 6.420
Bacillus cereus TKU028 - 0.127 + 0.018 0.111 + 0.018 0.776 + 0.053 68.72 + 5.143
Bacillus mycoildes TKU038 - - 0.101 + 0.017 0.463 + 0.036 111.67 + 1.036
Bacillus mycoildes TKU040 - 0.191 + 0.020 - - -
Rhizobium sp.TKU041 - - - - -

deCSP was used at the sole C/N source with a concentration of 1% (w/v). Culture conditions were set at 37 °C, 150 rpm shaking speed, 100/250 mL (volume of medium/flask) for two days. The cultures of fermented deCSP were obtained and centrifuged at 12,000× g for 20 min to collect the culture supernatants. (-) expressed activity lower than 0.1 U/mL and 10 U/mL for exochitinase and αGI, respectively.